My Little Greenhouse

BatCave

BatCave

Well-Known Member
Someguy15 said:
God I can't wait to fruit! lol knocking up 8 more jars. 4 Albino A+ and 4 Texas. Those look amazing dude, think you will get a third flush?
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Yes it should have 1 more flush left in it. Might clone the big one, just waiting to see how it finishes
 
Someguy15

Someguy15

Well-Known Member
When u take a tissue sample it's not actually an isolation tho right? I've really been wanting to get into agar and master culture slants but looks to b about $150 in supplies to do it. Eh probably soon enough, I love mad scientist shit lol
 
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DaSprout

Well-Known Member
Looks like you really have a good rotation going down. That's nice to see for at home styles. That's something that I'm currently working for.
 
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testtime

Well-Known Member
Someguy15 said:
When u take a tissue sample it's not actually an isolation tho right? I've really been wanting to get into agar and master culture slants but looks to b about $150 in supplies to do it. Eh probably soon enough, I love mad scientist shit lol
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Tissue samples from a single shroom is an unknown. It could be a mosaic, or it could be a single isolate, no way to tell (at least for me).

But that being said, I would much rather limit it down to the smallest isolated bit from a cap than have to run through dozens of agar isolates before finding one that had the characteristics I want. Agar work requires a special kind of patience (hehe, that word again) that I do not have, at least no so far.

One the other hand, if you have a particular bit of genetics you want to save, agar work is pretty much the only way, so start learning if that is your goal.
 
canndo

canndo

Well-Known Member
testtime said:
Tissue samples from a single shroom is an unknown. It could be a mosaic, or it could be a single isolate, no way to tell (at least for me).

But that being said, I would much rather limit it down to the smallest isolated bit from a cap than have to run through dozens of agar isolates before finding one that had the characteristics I want. Agar work requires a special kind of patience (hehe, that word again) that I do not have, at least no so far.

One the other hand, if you have a particular bit of genetics you want to save, agar work is pretty much the only way, so start learning if that is your goal.
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\

Well, I posted a primer on agar work, of course half of it dissapeared but the first half might help. I don't agree with the 150 dollar total though, I think if you have a glove box you can do it all for about 70.
 
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testtime

Well-Known Member
canndo said:
\

Well, I posted a primer on agar work, of course half of it dissapeared but the first half might help. I don't agree with the 150 dollar total though, I think if you have a glove box you can do it all for about 70.
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Oh, I'll be playing with it soon enough. At least I think so. About a year ago I got "done". I just got a shipment of petri dshes, sleeves, and a variety of agar formulas. I had PLANS! But I got tired. I had too much too do in the real world, and the closet wasn't as fun any more.

I ended up trashing 20 trays, in various stages of growth, just to be done. So the agar sat. Many jars sitting. I wonder what will happen when they are opened.

Well, it seems I'll have space and time and a newfound interest, that'll come together in about a month. So back for a bit for play and learning.
 
Someguy15

Someguy15

Well-Known Member
canndo said:
\

Well, I posted a primer on agar work, of course half of it dissapeared but the first half might help. I don't agree with the 150 dollar total though, I think if you have a glove box you can do it all for about 70.
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yeah sorry that included some ca bicarb, 24 synthetic filter discs, 10 syringes, 24 test tubes for slants and autoclavable flask, you can use a alch fifth as a cheap replacement I guess.
 
canndo

canndo

Well-Known Member
BatCave said:
I have a nice vacuum sealer but never break it out anymore, I have had good of luck with stapeling and clothes pins.
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Hey Bat, I rushed in here to delete that post - a guy on another post pointed out that I am being a dick - and he is right, Too late though you read it already. I don't mean to be telling everyone who have had great sucess always something better. Excuse me for doing that please?
 
BatCave

BatCave

Well-Known Member
canndo said:
Hey Bat, I rushed in here to delete that post - a guy on another post pointed out that I am being a dick - and he is right, Too late though you read it already. I don't mean to be telling everyone who have had great sucess always something better. Excuse me for doing that please?
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Its all good man, I know you mean no harm and are just trying to show another way of doing things, the actual proper and safest way of doing it. I have just been exceptionally lucky with my laziness in shortcuts.

No harm no foul and keep the info coming.
 
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