look at post 13
and i think with the application of certain hormones,shoots can develope....i.e. tissue culture
In tissue culture, plants can be created from leaves but it is best when those leaves have been produced themselves in tissue culture. the auxins in the medium seem to create callus tissue and roots in the ordinary course of TC cloning. Sterilizing leaves to put in tissue culture most often kills the porous tissue. Also 1-1/2" branch tips make best starts for Microclone-style TC. Here are a few photos of callus and root leaves just seen in Dec.
