Root Clone Test - pictures
- Thread starter Hobbes
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Jester88
Well-Known Member
id think the main mass would have to stay as one big living structure. I don't think that the roots with survive in segments. Though there is a good chance that I could be wrong, coz it's plenty for other plants
My views are that the roots need to be left as a living structure so the re-vegging thing might work.
From what I gather, what we want to achieve here is for a plant to grow foliage from the root system kapeesh. again like grape vines and roses but in a controllrd environment to do this the best results would be achieved with hormones or as a living deep tissue culture as such but yeah i just rote a big thing only to lose half of it, in short
i think the roots have to be expoed to the right environment as i said earlier in the thread and the growth is more likely to come from the laateral or or fiborous and advantitious roots at the surface is my oppinion possible even on the ends kinda like grape vines, if you leave some growth perhaps forced growth is possible like when roses shoot out from the bottom of the plant. they build up the chemicals they need and then make a place to force shoot from more or less. so yeah by leaving some you may get more growth or at least more clones if it does reveg.... then you get lots again though they may lose some potency and vigour the genetics are the same and can still be bred with.. i just wouldnt if i could avoid it, though sometimes its gotta be done. the less stress the better imho.
anyhoo ill try again btw this is a rough example and first pics i found (for the second time lol) anyhoo
example of roots
now my idea is leave them close to the surface ofthe ground hell let some see the surface. see if they start producing the auxins and chemicals to make a calys and shoot from it. perhaps this is the way people need to go.
again im sorry this is nothing com[pared to ma first post that ws ruined but i re writ the bulk of it anyhoo lol.
peace out
jester
My views are that the roots need to be left as a living structure so the re-vegging thing might work.
From what I gather, what we want to achieve here is for a plant to grow foliage from the root system kapeesh. again like grape vines and roses but in a controllrd environment to do this the best results would be achieved with hormones or as a living deep tissue culture as such but yeah i just rote a big thing only to lose half of it, in short
i think the roots have to be expoed to the right environment as i said earlier in the thread and the growth is more likely to come from the laateral or or fiborous and advantitious roots at the surface is my oppinion possible even on the ends kinda like grape vines, if you leave some growth perhaps forced growth is possible like when roses shoot out from the bottom of the plant. they build up the chemicals they need and then make a place to force shoot from more or less. so yeah by leaving some you may get more growth or at least more clones if it does reveg.... then you get lots again though they may lose some potency and vigour the genetics are the same and can still be bred with.. i just wouldnt if i could avoid it, though sometimes its gotta be done. the less stress the better imho.
anyhoo ill try again btw this is a rough example and first pics i found (for the second time lol) anyhoo
example of roots
now my idea is leave them close to the surface ofthe ground hell let some see the surface. see if they start producing the auxins and chemicals to make a calys and shoot from it. perhaps this is the way people need to go.
again im sorry this is nothing com[pared to ma first post that ws ruined but i re writ the bulk of it anyhoo lol.
peace out
jester
J.cun.Shallow
Active Member
without bein a dick about it, when can we say that it dont work.
Hobbes
Well-Known Member
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We cannot say that root cloning does not work, only that we haven't been able to make it work so far. Takes time. I'm still harvesting roots from most plants, using zip lock bags so I can keep a stack of root bags in one bucket.
Break out the rocking chair, fire up your vaporizer.
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LOL! A fair question.
We cannot say that root cloning does not work, only that we haven't been able to make it work so far. Takes time. I'm still harvesting roots from most plants, using zip lock bags so I can keep a stack of root bags in one bucket.
Break out the rocking chair, fire up your vaporizer.
.
mcpurple
Well-Known Member
root cloning does work i had a bunch of roots fro some strawberries and put them in dirt and bam new roots and a new plant just look it up its been doen has been done for years its just marijauna is a harder plant to do it to. strawberries almost do it naturally all by them selfs along with alot of other plants
That 5hit
Well-Known Member
lol .
v12xjs
Well-Known Member
I never got the time to do anything with my last plant but it occurred to me that even people who clone use a chemical stimulant to induce rooting, so rather than mess around with unknown methods I looked into that. It seems that the vegetative equivalent of rooting hormone is called cytokinins. I haven't had any time to find out how easily available they are but the discovery came about as a result of a guy in a lab in the 1940's who spotted that his plants grew bigger flowers when he added raw coconut water to them. It turns out coconut water is full of cytokinins which seem to encourage cell division.
I'm studying for exams right now so I can't really do much with it but I'd love someone to follow this up and see how easy it is to get the pure cytokinins.
I'm studying for exams right now so I can't really do much with it but I'd love someone to follow this up and see how easy it is to get the pure cytokinins.
That 5hit
Well-Known Member
Nature of Cytokinins
Cytokinins are compounds with a structure resembling adenine which promote cell division and have other similar functions to kinetin. Kinetin was the first cytokinin discovered and so named because of the compounds ability to promote cytokinesis (cell division). Though it is a natural compound, It is not made in plants, and is therefore usually considered a "synthetic" cytokinin (meaning that the hormone is synthesized somewhere other than in a plant). The most common form of naturally occurring cytokinin in plants today is called zeatin which was isolated from corn (Zea mays).
Cytokinins are compounds with a structure resembling adenine which promote cell division and have other similar functions to kinetin. Kinetin was the first cytokinin discovered and so named because of the compounds ability to promote cytokinesis (cell division). Though it is a natural compound, It is not made in plants, and is therefore usually considered a "synthetic" cytokinin (meaning that the hormone is synthesized somewhere other than in a plant). The most common form of naturally occurring cytokinin in plants today is called zeatin which was isolated from corn (Zea mays).
Cytokinins have been found in almost all higher plants as well as mosses, fungi, bacteria, and also in tRNA of many prokaryotes and eukaryotes. Today there are more than 200 natural and synthetic cytokinins combined. Cytokinin concentrations are highest in meristematic regions and areas of continuous growth potential such as roots, young leaves, developing fruits, and seeds (Arteca, 1996; Mauseth, 1991; Raven, 1992; Salisbury and Ross, 1992).
History of Cytokinins
In 1913, Gottlieb Haberlandt discovered that a compound found in phloem had the ability to stimulate cell division (Haberlandt, 1913). In 1941, Johannes van Overbeek discovered that the milky endosperm from coconut also had this ability. He also showed that various other plant species had compounds which stimulated cell division (van Overbeek, 1941). In 1954, Jablonski and Skoog extended the work of Haberlandt showing that vascular tissues contained compounds which promote cell division (Jablonski and Skoog, 1954). The first cytokinin was isolated from herring sperm in 1955 by Miller and his associates (Miller et al., 1955). This compound was named kinetin because of its ability to promote cytokinesis. Hall and deRopp reported that kinetin could be formed from DNA degradation products in 1955 (Hall and deRopp, 1955). The first naturally occurring cytokinin was isolated from corn in 1961 by Miller (Miller, 1961). It was later called zeatin. Almost simultaneous with Miller Letham published a report on zeatin as a factor inducing cell division and later described its chemical properties (Letham, 1963). It is Miller and Letham that are credited with the simultaneous discovery of zeatin. Since that time, many more naturally occurring cytokinins have been isolated and the compound is ubiquitous to all plant species in one form or another (Arteca, 1996; Salisbury and Ross, 1992).
Biosynthesis and Metabolism of Cytokinins
Cytokinin is generally found in higher concentrations in meristematic regions and growing tissues. They are believed to be synthesized in the roots and translocated via the xylem to shoots. Cytokinin biosynthesis happens through the biochemical modification of adenine. The process by which they are synthesized is as follows (McGaw, 1995; Salisbury and Ross, 1992):
A product of the mevalonate pathway called isopentyl pyrophosphate is isomerized.
This isomer can then react with adenosine monophosphate with the aid of an enzyme called isopentenyl AMP synthase.
The result is isopentenyl adenosine-5'-phosphate (isopentenyl AMP).
This product can then be converted to isopentenyl adenosine by removal of the phosphate by a phosphatase and further converted to isopentenyl adenine by removal of the ribose group.
Isopentenyl adenine can be converted to the three major forms of naturally occurring cytokinins.
Other pathways or slight alterations of this one probably lead to the other forms.
Degradation of cytokinins occurs largely due to the enzyme cytokinin oxidase. This enzyme removes the side chain and releases adenine. Derivitives can also be made but the pathways are more complex and poorly understood.
Cytokinin Functions
A list of some of the known physiological effects caused by cytokinins are listed below. The response will vary depending on the type of cytokinin and plant species (Davies, 1995; Mauseth, 1991; Raven, 1992; Salisbury and Ross, 1992).
- Stimulates cell division.
- Stimulates morphogenesis (shoot initiation/bud formation) in tissue culture.
- Stimulates the growth of lateral buds-release of apical dominance.
- Stimulates leaf expansion resulting from cell enlargement.
- May enhance stomatal opening in some species.
- Promotes the conversion of etioplasts into chloroplasts via stimulation of chlorophyll synthesis
Total Head
Well-Known Member
so i dug up this thread after reading another thread about what sounded like accidental root cloning and would like to bump it up. as someone else mentioned, there is probably just something everyone is missing when doing these experiments. however in the thread i read (https://www.rollitup.org/general-marijuana-growing/189531-multiple-root-systems-one-seed.html, no pics) the poster claimed to have leaves in a few days, however this was done from a root from a pre-seedling. could it be possible that the fact that everyone is waiting till the end of harvest to take roots is what is slowing the process? if the account in that thread is correct and these roots can be cut from the single taproot that emerges from the seed, then we are right back in the realm of practicality because you could potentially take these clones before you plant the seed...i have read too much to down this idea. i am very much excited about it. i'm looking for a little bag of bagseeds i have somewhere so i can try this out myself.
Hobbes
Well-Known Member
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"could it be possible that the fact that everyone is waiting till the end of harvest to take roots is what is slowing the process?"
Total Head you are absolutely brilliant!! That is exactly what I did the first time around, I remember digging down and pulling out the roots and filling the hole back up with fresh Pro Mix.
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"i'm looking for a little bag of bagseeds i have somewhere so i can try this out myself."
I wish I could give you a couple of plants to run to get this going right away. If there's anything that I can do to help please let me know. I'm going to run some roots from plants 2, 4, 6, etc weeks of flower to see if a particular week works best, like green pulpy stems for cloning.
ITS ON AGAIN BABY!
,
"could it be possible that the fact that everyone is waiting till the end of harvest to take roots is what is slowing the process?"
Total Head you are absolutely brilliant!! That is exactly what I did the first time around, I remember digging down and pulling out the roots and filling the hole back up with fresh Pro Mix.
.
"i'm looking for a little bag of bagseeds i have somewhere so i can try this out myself."
I wish I could give you a couple of plants to run to get this going right away. If there's anything that I can do to help please let me know. I'm going to run some roots from plants 2, 4, 6, etc weeks of flower to see if a particular week works best, like green pulpy stems for cloning.
ITS ON AGAIN BABY!
,
Total Head
Well-Known Member
i found the bagseeds. i have a bunch of breeder seeds but i'd rather use "disposable" seeds for this. i have them germing on my cable box right now. after they are planted i will put some under the lamp and some i will put where the light meter barely registers, probably under my plant shelf. i see no reason why the idea of root cloning shouldn't be explored, and seed roots should have different levels of hormones than matured roots. i am certain that if my experiment fails it is my own fault. like anything, it probably takes some dialing in.
v12xjs
Well-Known Member
Plants just need a chemical stumulant to promote growth from roots.
This site used to sell the chemical product required to stimulate growth from roots. I can't remember what it was called but their supplier stopped production recently.
http://www.super-grow.biz/IAA.jsp
keep an eye on them because I'd bet they find another supplier soon.
This site used to sell the chemical product required to stimulate growth from roots. I can't remember what it was called but their supplier stopped production recently.
http://www.super-grow.biz/IAA.jsp
keep an eye on them because I'd bet they find another supplier soon.
mcpurple
Well-Known Member
my buddy said he had some plants in rockwool cubes and the roots grew out of them and into the water trey he said they turned green and after awhile started making new plants off the roots while the other plant was still growing. the roots were in water almost all the time. i really dont see why this is a big topic root cloning is done all the time
Total Head
Well-Known Member
this will be a kind of radical experiment (at least i think so). i have germed some nice bagseeds that i've been saving for god knows what 
and today they all had decent tails that were starting to get that "fur" where the rest of the roots will form. so i took my surgical scissors and sterilized them. i then selected the 3 seeds that appeared to have the most aggressive rooting (these seeds took 3 whole days to get tails this long, so this was as long as i was going to wait). on one of them i cut the furry tip completely off. on another i cut the tip off about halfway down the furry part. i did this on the third seed as well. i planted the tips as far apart from each other as i could in a red 4 inch container, and i planted their other halves in a green 4 inch container. i had origionally planned on individual containers for all but alas i am just about out of soil. the containers are way underfilled but this is just an experiment. i have at least 20 more of these seeds so i'm not sweating it. i altered a peice of clip art to show what i did in case i wasn't clear enough. it doesn't show the fur but you get the idea. i also included a pic of what the seeds looked like germed in the paper towel after 3 days. some of the slowest i have seen but the seeds are over a year old and the smoke was killer. no pics of after i cut them but i didn't want them exposed for long. they are currently in the plant room on veg cycle under hps. i've never had a problem getting seeds to pop under intense light before. let's do this.
and today they all had decent tails that were starting to get that "fur" where the rest of the roots will form. so i took my surgical scissors and sterilized them. i then selected the 3 seeds that appeared to have the most aggressive rooting (these seeds took 3 whole days to get tails this long, so this was as long as i was going to wait). on one of them i cut the furry tip completely off. on another i cut the tip off about halfway down the furry part. i did this on the third seed as well. i planted the tips as far apart from each other as i could in a red 4 inch container, and i planted their other halves in a green 4 inch container. i had origionally planned on individual containers for all but alas i am just about out of soil. the containers are way underfilled but this is just an experiment. i have at least 20 more of these seeds so i'm not sweating it. i altered a peice of clip art to show what i did in case i wasn't clear enough. it doesn't show the fur but you get the idea. i also included a pic of what the seeds looked like germed in the paper towel after 3 days. some of the slowest i have seen but the seeds are over a year old and the smoke was killer. no pics of after i cut them but i didn't want them exposed for long. they are currently in the plant room on veg cycle under hps. i've never had a problem getting seeds to pop under intense light before. let's do this.Attachments
Total Head
Well-Known Member
all right so 2 days ago i planted the different halves in separate pots, and so far the "seed end" of my seedlings are poking up through the soil. the pic below was actually taken yesterday and since then the other 2 sprouts have come up. the root tip pot has shown nothing, but i assume building a set of primaries takes a bit longer than simply lenthening the root, so i'm still hopeful this will work. if anything i've proven that the idea of treating freshly germed seeds with kid gloves is a lot of hooey. i manhandled these things and chopped off their tips and they grow anyway, seemingly at the same rate as any other seed i've planted. my theory is that the part i chopped off will in fact sprout 2 root tips and perhaps grow faster than it would have otherwise. to clarify the seedling in the pic is to the right and a little below center. it's small but it's there. i will put up a pic tomorrow of the other 2 that came up. could take a bit for the tips to come up though.
