Grain bag showing no growth after 3 weeks

HeatlessBBQ

HeatlessBBQ

Well-Known Member
You say the 3rd of this Month, Mate?

If so; since it has been 3 weeks, there should have been sign of colonization
around day 5 after inoculation.

You should bin the bags after the bags are 85%-95% colonized.
 
TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
HeatlessBBQ said:
Excuse Me, Mate.

Thought You were speaking of a bulk method.

Going to use the rest of that 5cc?
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What? This doesn't even make sense dude. Bulk method, what? I had 2 grain bags, they haven't colonized at all in 3 weeks. Should I throw them out or keep waiting? I don't get what's unclear here
 
Voodu

Voodu

Well-Known Member
TheGrassIsGreenerInAus said:
OK but still, nobody is answering the meat of my question should I throw them out????
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If you look into the bags do you see any signs of colonization? Do they smell bad or have any color to them that is not white? What are the conditions like in the room you're doing this? What temperature and humidity do you maintain? How exactly did you go about innoculating your bags? What type of spore strain are you using? Where did you get the spores from? How did the spore syringe look before you used it? Can you post some pictures or provide more information?
 
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TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
Voodu said:
If you look into the bags do you see any signs of colonization? Do they smell bad or have any color to them that is not white? What are the conditions like in the room you're doing this? What temperature and humidity do you maintain? How exactly did you go about innoculating your bags? What type of spore strain are you using? Where did you get the spores from? How did the spore syringe look before you used it? Can you post some pictures or provide more information?
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Um wow. I kept them in the Laundry with a heater going, first few days could have been too hot before I got my thermometer out there but once it was it sat between 20-26C so right in the good spot I think. There's no signs of any color change, no smells I've noticed, nothing. They just look the same as when I got them. I got the syringe from mushroomprints.com. Golden teachers I believe.
 
Voodu

Voodu

Well-Known Member
I am no expert on this subject, but, I know you need to have your substrate moist and sterilized before you innoculate. I assume your grow bag is sterile since it's store bought, but was it bone dry when you put in the spore solution?

Also, those little black puck looking things, they are activated heaters, did you do anything to activate them? Or did the bags come with any instructions?
 
TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
Voodu said:
I am no expert on this subject, but, I know you need to have your substrate moist and sterilized before you innoculate. I assume your grow bag is sterile since it's store bought, but was it bone dry when you put in the spore solution?

Also, those little black puck looking things, they are activated heaters, did you do anything to activate them? Or did the bags come with any instructions?
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I bought them online actually but yes they were pre sterilized lol. Um they were most definitely NOT bone dry, I was under the impression that that was right too, mycelium requires moisture to grow
 
TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
Voodu said:
I am no expert on this subject, but, I know you need to have your substrate moist and sterilized before you innoculate. I assume your grow bag is sterile since it's store bought, but was it bone dry when you put in the spore solution?

Also, those little black puck looking things, they are activated heaters, did you do anything to activate them? Or did the bags come with any instructions?
Click to expand...
And from what I read online, the black pucks were the self sealing hole to inoculate through.
 
Voodu

Voodu

Well-Known Member
It looks to me like you messed something up then. I am not going to presume what precisely it was. Based on the information you have provided me, my suggestion (for what it;'s worth) is to get rid of them and start again. It looks like 0 colonization has taken place.
 
TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
Voodu said:
It looks to me like you messed something up then. I am not going to presume what precisely it was. Based on the information you have provided me, my suggestion (for what it;'s worth) is to get rid of them and start again. It looks like 0 colonization has taken place.
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Yeah that's what I thought =( gonna try a couple pf tek Jars with the last 5ml in my syringe, if THEY don't grow then clearly the syringe was effed
 
TheGrassIsGreenerInAus

TheGrassIsGreenerInAus

Well-Known Member
I'll leave em out there a while but just in case haha I read a story online about a guy who tried to colonize a jar 2 weeks and nothing so it wound up in a bag forgotten, pulled out a month later fully colonized =)
 
HeatlessBBQ

HeatlessBBQ

Well-Known Member
The substrate is very prone to contaminates when inoculated more than once.
That is why it is recommended with bags to use the whole syringe per penetration.

Even if You use a brand new syringe, the bags have open holes in them.
If You did not seal with micropore tape the bags are already technically contaminated.
Especially if You do another inoculation and if You do not sterilize the needle before.
 
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HeatlessBBQ

HeatlessBBQ

Well-Known Member
I have gotten contaminates every single time I reused a syringe AND needle.

PF Tek or Bulk Bags. Even agar and rye jar teks can easily get contaminated.
 
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