technical dan
Active Member
ok update time the first cube jar (one I pulled out of the inc.) is slowly growing after shaking/ rolling the jar the other day. And then Jar two is going:
second pic was taken today and the first was taken on the 22nd. I think I'm going to let that spot (only one I've seen) grow a bit more and then roll/ shake the jar. The light white on the right is because of the camera and lighting it is not actually in the jar.
I have not seen anything in any of the P. galindoi jars (two sets of inoculations and 6 pint jars total). Today I saw some white growth on various areas of the agar jar with GT . Nothing I can see on the glove box agar jar and now I have some oyster tissue on an agar jar. I have 3 pints with popcorn ready to be PC'ed going to do the first 2 tonight (hopefully). Two have silicone port lids and the third does not so the two will be shot up with GT and hopefully things will continue happening on the agar so I can transfer whatever (GT or oyster) happens to be ready into the third jar sometime soon.
I also had a complication with the tissue transfer. I had a flash fire in my glove box, which luckily stayed sealed so the fire ran out of oxygen and went out in under a second without harming myself or anything else (except the glove box lid). I was lazy and impatient and had started spraying the inside of the box of bleach solution did not have enough left so after letting it sit I sprayed my gloves and jar with iso alcohol ( up to then I had only used as little as needed to sanitize my gloves). So I had built up a high enough concentration of vapors to ignite when I used the lighter to flame sterilize my exacto. I had considered the possibility of a fire due to iso before this happened so as soon as the lighter lit and the flame was 3 times larger than it should be I knew what was happening and was pulling my hands out. After taking it outside and trying to open the box I saw the fire sealed the lid on (melting/ welding it in some places) everything was still in there so I went ahead and did the transfer to agar. I need to replace the lid for my box after having to pry and break bits of it off to remove it, but other than that everything is ok.
So my impatience with not wanting to have to open, spray, and sit my box again lead to a lapse in judgement which bit me in the ass less than 5 minutes later. Hopefully this will serve as a reminder to everybody as it will to me to do all the steps and think shit through.
second pic was taken today and the first was taken on the 22nd. I think I'm going to let that spot (only one I've seen) grow a bit more and then roll/ shake the jar. The light white on the right is because of the camera and lighting it is not actually in the jar.
I have not seen anything in any of the P. galindoi jars (two sets of inoculations and 6 pint jars total). Today I saw some white growth on various areas of the agar jar with GT . Nothing I can see on the glove box agar jar and now I have some oyster tissue on an agar jar. I have 3 pints with popcorn ready to be PC'ed going to do the first 2 tonight (hopefully). Two have silicone port lids and the third does not so the two will be shot up with GT and hopefully things will continue happening on the agar so I can transfer whatever (GT or oyster) happens to be ready into the third jar sometime soon.
I also had a complication with the tissue transfer. I had a flash fire in my glove box, which luckily stayed sealed so the fire ran out of oxygen and went out in under a second without harming myself or anything else (except the glove box lid). I was lazy and impatient and had started spraying the inside of the box of bleach solution did not have enough left so after letting it sit I sprayed my gloves and jar with iso alcohol ( up to then I had only used as little as needed to sanitize my gloves). So I had built up a high enough concentration of vapors to ignite when I used the lighter to flame sterilize my exacto. I had considered the possibility of a fire due to iso before this happened so as soon as the lighter lit and the flame was 3 times larger than it should be I knew what was happening and was pulling my hands out. After taking it outside and trying to open the box I saw the fire sealed the lid on (melting/ welding it in some places) everything was still in there so I went ahead and did the transfer to agar. I need to replace the lid for my box after having to pry and break bits of it off to remove it, but other than that everything is ok.
So my impatience with not wanting to have to open, spray, and sit my box again lead to a lapse in judgement which bit me in the ass less than 5 minutes later. Hopefully this will serve as a reminder to everybody as it will to me to do all the steps and think shit through.