My first class with the golden teacher

G

growyurown

Well-Known Member
polyarcturus said:
i think he would have to build a set of mechanical eyes to roll them at all the shit we say :D
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Lol yea. Ive asked him a million questions and hes always happy to help. Good guy with knowledge. But anyways i hope I didnt fuck up by putting too much corn in my jars (7/8) or so but he will surely chime in.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
i dont think you can put too much in....a little less than full is good though because it allows you to shake them and ditribute the colonized kernels around better than a full jar.

i also noticed that they expand a little in the pressure cooker so around 3/4 of the way is about good before PCing...a little less isnt bad either...

if your doing like i am and spawning to a bulk sub then a little bit of corn goes a long way, once the mycelium start colonizing that bulk substrate it takes off pretty fast. i used 1 1/2 pint jar of corn per tray and i feel i could have gotten away with way less....

the more corn though the faster your going to colonize your bulk sub....just because theres more surface are of mycelium to spread.

this is just my 2¢ in my experience. i may not be 100% accurate but i cant be completly wrong either lol
 
G

growyurown

Well-Known Member
When it does fully colonize how do you get the mycelium broken up without damaging it? I shook my jars but the colinized areas are clumps
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
woot my bud just texted me and said he seen about a dozen pins and was sure that they were pins...heading over in a min with the ole camera to take some shots!!!

so excited lol
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
growyurown said:
When it does fully colonize how do you get the mycelium broken up without damaging it? I shook my jars but the colinized areas are clumps
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just shake em up or break em apart. i removed my corn as a "cake" and just broke it up into the straw.. it does damage it a little but it repairs itselfquick if its strong.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
and its confirmed...i have pinning in my verm/popcorn tray (the first one i set out to fruit)

IMG_5379.jpgIMG_5382.jpgIMG_5383.jpgIMG_5384.jpgIMG_5380.jpgIMG_5381.jpg


so i set out the 4 cased pans to fruit. only had enough perilite to do one of the FC's so i used vermiculite for the other....figure they serve the same general purpose which is to retain moisture to increase the humidity.

IMG_5385.jpgIMG_5386.jpg

and heres a couple more pics of the rest of the setup

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technical dan

technical dan

Active Member
Looks nice matt!

Today I saw that I have white mycelium growing in my second P. cube pint. But then I also saw that I have rust colored bacterial looking contamination in the first P. cube pint and in my 1/2 pint P. galindoa (no visible myc.) and then some kind of myc. that is not P. cube in my P. cube half pint. I took all the contam jars out of the incubator so the contams will not grow as quickly.... but these jars should just be tossed now or should I keep them in the cooler conditions and see if the shroom mycelium wins out? I already threw the P. cube 1/2 pint away since there was visible contam and no visible myc. growth.
 
Javadog

Javadog

Well-Known Member
Congrats on the new fruits.

I am sure that more are about to pop up.

Have fun.

JD

P.S. There is no need to throw any lost jars away. You may want to re-boil or PC
them to make sure that you do not spread mold spores. I have kept some older
jars, just to see what would win. The result is almost always tossing the jar at
some point, and even if the mushroom appears to win, there is likely a hidden
contam in the spawn that will show itself once the sub gets tired. You can still
get flushes though. Good luck!
 
polyarcturus

polyarcturus

Well-Known Member
just threw a vid up of me doing an isolation on agar and inoc some grain with agar for g2g on m thread. check it out when you get a chance guys.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
Thundercat said:
Yay pins now it gets fun!
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yeah im hoping by end of week i can harvest some fruits.....im just anxious to watch them grow. once i get stuff fruiting fully i think my next grow will be better since i will have that impatience to fruit out of the way....i really should have waited on fruiting the 2 SGFC's with the peat/verm casing untill i seen a even mycelium spread across the top of the casing but i dont want to stagger everything too much. from now on though ill be doing 5 pans at a time and using the 2 larger totes and the one smaller tote. anyways live and learn...im pretty sure now ill get a decent harvest. if that pan of just colonized popcorn and plain vermiculite will fruit im sure the straw bulk will

technical dan said:
Looks nice matt!

Today I saw that I have white mycelium growing in my second P. cube pint. But then I also saw that I have rust colored bacterial looking contamination in the first P. cube pint and in my 1/2 pint P. galindoa (no visible myc.) and then some kind of myc. that is not P. cube in my P. cube half pint. I took all the contam jars out of the incubator so the contams will not grow as quickly.... but these jars should just be tossed now or should I keep them in the cooler conditions and see if the shroom mycelium wins out? I already threw the P. cube 1/2 pint away since there was visible contam and no visible myc. growth.
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sorry cant like this post...too many mentiones of contams....hope the couple jars that have mycelium in the work out well. feel free to post pics if you like
 
technical dan

technical dan

Active Member
Thank you. Yep there is more contam than I hoped to see and if it keeps showing up in my other jars then I deff. have a problem somewhere. When Im at the store next I'm going to look to see if they have the stuff for a homemade agar tek. It seems a bit early for me to be jumping in to that (esp. since I dont even have full colonization, and have never fruited/ very little exp) but agar really looks like the way to go.

My camera is not good and has IMO questionable focus points but I took pics of a couple jars and circled stuff with paint. Mycelium is in blue circles and rust bacteria (?) in the orange area. The bacterial colonies are hard to find in person and nonexistent in the pic but the circling and marks show the area they are in.

jar1contammarked.jpgjar2marked.jpg
 
MJG420

MJG420

Well-Known Member
RetiredMatthebrute said:
just shake em up or break em apart. i removed my corn as a "cake" and just broke it up into the straw.. it does damage it a little but it repairs itselfquick if its strong.
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Not sure about this..... seems something on my end has gone horribly wrong! Things were looking great on Friday and I gave all the jars a good shaking, checked them yesterday and nothing....
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
i have been shakin the rat piss out of mine to mix up the colonized kernels as much as possible and withing 2-3 days i see new mycelium growing.

what did you say your temps were? may be on the low side so growth is slower.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
technical dan said:
Thank you. Yep there is more contam than I hoped to see and if it keeps showing up in my other jars then I deff. have a problem somewhere. When Im at the store next I'm going to look to see if they have the stuff for a homemade agar tek. It seems a bit early for me to be jumping in to that (esp. since I dont even have full colonization, and have never fruited/ very little exp) but agar really looks like the way to go.

My camera is not good and has IMO questionable focus points but I took pics of a couple jars and circled stuff with paint. Mycelium is in blue circles and rust bacteria (?) in the orange area. The bacterial colonies are hard to find in person and nonexistent in the pic but the circling and marks show the area they are in.

View attachment 2576531View attachment 2576532
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that second jar looks promising. all you need is one good jar with grain and your good. g2g transfers arent as complex as they seem and once you have fully colonized mycelium going into a new jar it takes hold fast...much faster than using a syringe. in my experience.

i really think having the temp swings from 74-84 in my incubator is contributing the my speeds though.
 
canndo

canndo

Well-Known Member
polyarcturus said:
View attachment 2571294

hey matt this is my agar jar that was successfully inoculated. basically since i didnt get any growth i would really like to see, so im not gonna be doing an isolation, plus its much too late for that. (cutting away a section of combined spore mycelium, i use a PCed exacto knife in a glove box and quickly transferred it.) and i plan on getting some more spores soon anyways. so what i will be doing instead is much like a G2G, i will take a jar of PCed popcorn and quickly pour it in and allow it to colonize the popcorn for more G2G. i could also add a sharp object and cut the mycelium up, add water and food and make an LC too, the options are limitless with agar.

hey canndo i got some jars fighting contam(5 to be exact) where the mycelium has a foot hold and is slowly overtaking the contam, anything i can do to salvage these? any idea for them, even outside.. or just toss them?
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Bury them in the ground. I did this with quick failed run of agaricus and got surprised yesterday as I was planting a tree with some fruit, you might have the same experience.
 
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